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I wander if SCIO is sensitive to illumination noise/variability or is it using lock-in amplification i.e. strobes excitation light to estimate actual emission spectra measured locked to the illumination strobe corrected by measured spectra obtained out-of-phase with the strobe, i.e. containing background illumination.
Does angle of scan contribute to the measurement variance ? I imagine reflectance spectrum in NIR is also angle dependent, isn’t it?
SCiO measures the ambient light and subtracts it from the signal. It does not a lock-in amplifier.
A for different angles, SCiO can be held at different angles towards the sample (see image below), but the best practice is to hold SCiO straight, facing the sample.